We are seeking a Research Specialist II who will work under the general supervision of a faculty investigator in the Division of Cardiology and will be responsible for independently planning and execuating projects in the investigation of the effect of various genetic mutations on thrombosis. They will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
Reports to: William Osburn.
Specific Duties & Responsibilities:
Follow established procedures and utilizes appropriate technique to perform experiments associated with investigating the role of candidate gene mutations in thrombosis and hemostasis. Includes, but not limited to:
Execute independent research projects involving gene expression, molecular cloning, and protein and cell-based assays and experiments using human endothelial cell lines and cancer cell lines.
Assist with or lead small animal model husbandry and experiments.
Be familiar with basic laboratory techniques such as pipetting, buffer and reagent preparation, pH determination, qPCR, RT-qPCR, western blotting, ELISA spectrophotometric absorbance determination and fluorescence detection.
Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, scintillation counter, light and/or fluorescence microscope, microplate reader, plate washer and biological safety cabinet.
This person may also utilize sophisticated instrumentation, spectrophotometer, thermal cycler, flow cytometer, and laboratory automation solutions/robotics.
Responsible for operation of lab computers for data entry.
Maintain accurate data records and laboratory notebook.
Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment.
Prepares solutions, reagents, and stains following standard laboratory formulas and procedures.
Along with the laboratory supervisor, works to develop and institute standard operating procedures.
Minimum Qualifications (Mandatory):
Bachelor's in Biochemistry, Molecular Biology, Cell Biology, Chemistry, Bioengineering or related field required. Master's Degree preferred.
2 years laboratory experience performing relevant techniques.
4-5 years of related work experience preferred.
* Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
Special Knowledge, Skills & Abilities:
Proficiency in standard biochemistry and cellular and molecular biology experimental techniques.
Proficiency in both written and verbal scientific communication.
Prior small animal research experience is a plus but not required.
Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $39,310 - $54,060 annually (commensurate with experience) Employee group: Full Time Schedule: Monday to Friday 8:30am to 5:00pm Exempt Status: Exempt Location: JH Downtown Center Department name: Cardiology Administration Personnel area: Johns Hopkins University
The successful candidate(s) for this position will be subject to a pre-employment background check.
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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