The Research Specialist will work in the Division of Pulmonary and Critical Care Medicine and will interface with members of this and other labs in the Division. The position will utilize a solid foundation in basic laboratory principles and techniques associated with molecular and cell biology to perform the required tasks. A qualified applicant should be capable of exercising independent judgment while participating in the planning of experiments and evaluating results. When needed, he or she will also provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
The Research Specialist will work in a laboratory which focuses on the mechanisms of airspace maintenance and injury with a focus on COPD and genetic matrix disorders. The Research Specialist will utilize a solid foundation in basic laboratory techniques to maintain laboratory equipment, reagent inventories, and assist in routine experimental work like plasmid preparations, and cell culture. The work will also involve preparing and maintaining cell lines stably with overexpressed or knockdowned target genes. The Research Specialist has experience with cell handling and is capable of exercising independent judgement while participating in the planning of experiments and evaluating and interpreting results.
Specific duties & responsibilities:
The Research Specialist will participate in planning studies and evaluating test results, utilizing detailed knowledge of research laboratory procedures and practices, involving primary cells, cultured cell lines and animal models. These lab-based duties will include:
Perform cell imaging studies including real-time approaches to evaluate the localization and behavior of wild-type and mutant receptors in fibroblast cell lines.
Quantitative analysis of mRNA and protein expression in cells and tissues.
Generating precision cut lung slices from animal models to examine pathophysiological changes during disease development.
Independent troubleshooting of technical and experimental issues
Selects, develops, performs and evaluates research methods; operates appropriate equipment.
Uses sterile techniques to avoid contaminating lab experiments. Be knowledgeable of and employ good tissue culture techniques.
Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
Is an integrated member of the research team. May be involved in establishing laboratory protocols. Will be asked to prepare reports on the analysis.
Exercises professional and scientific judgment and assumes responsibility for decisions, consequences, and results. Engages in regular and continuous decision making having an impact on the success of the laboratory.
Exchanges routine information in an appropriate manner requiring good oral and written communication skills. Has operating knowledge of the personal computer and has the ability to search and retrieve information on the role of extracellular matrix proteins in inflammation and fibrosis from the internet.
Any specific physical requirements for the job:
Sitting in normal seated position for extended periods of time. Ability to use a keyboard and move about. Ability to see and hear within normal parameters.
Bachelor's degree in biology, chemistry or related field
One year of laboratory experience performing relevant techniques
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula
Master's degree in the related field.
Classified Title: Research Specialist Working Title: Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: 34,560 - 47,490 - 60,410 Salary (Commensurate with experience) Employee group: Casual / On Call Schedule: Varies Exempt Status: Non-Exempt Location: School of Medicine - East Baltimore Campus Department name: 10002817-SOM DOM Pulmonary Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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