The laboratory studies the basic mechanisms regulating myocardial angiogenesis and right ventricular remodeling in pulmonary hypertension. The Research Technologist will perform and analyze an array of experiments using isolated endothelial cells in culture and tissue samples obtained from animal models of pulmonary hypertension and patients with pulmonary hypertension. Requires a proficient Research Technologist with a sophisticated understanding of techniques and a solid foundation in the underlying science gained from education and personal experience. Participates in the design of experiments, analyzing data, presentation at lab meetings, and may contribute to manuscripts. Will provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
Essential Job Functions:
Maintenance of mouse colonies; genotyping
Harvesting of tissues from mice; previous exposure to harvest of lung tissue is preferred.
Cell culture and maintenance. Must be knowledgeable of good tissue culture techniques and basic principles of light microscopy; previous work with endothelial cells is a bonus
Must be proficient in basic molecular biology techniques, including protein/DNA/RNA extraction and quantification from cells and tissues, Western blotting, ELISA, quantitative PCR
Immunostaining and immunofluorescence microscopy. Previous exposure to basic image analysis programs (Image J) preferred.
Preparation and staining of cells for flow cytometry analysis
Analyzes data, formulates conclusions, and creates appropriate statistical presentations, including graphs and charts for the purpose of reporting research/laboratory results and quality control. Attend and present data in weekly lab meetings.
Conduct literature searches; work with PI and other lab members on manuscript preparation
Independent troubleshooting of technical and experimental issues
Basic lab management responsibilities, including preparing stock solutions, buffers and media, upholding safety regulations, maintaining records, and managing supplies
Special Knowledge, Skills, and Abilities:
Exchanges routine and special information in an appropriate manner requiring good oral and written communication skills.
Work produced is subject to precise measures of quantity and quality
Work environment may include areas of unpleasant extremes of cold or heat
Biohazardous conditions such as the risk of fumes, toxic or caustic chemicals, and human cells or tissues are present in this work environment, which mandate attention to safety considerations.
Near vision to see objects clearly within 20 inches
Sharp focus to adjust vision when doing close work that changes in distance from eyes
Full spectrum vision to identify and distinguish color
Handling by seizing, hold, grasping, turning, or otherwise working with the hand or hands but without finger dexterity
Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s); for example, using a keyboard
Communication skills using the spoken word
Occasionally lifting and carrying objects weighing 10 pounds or less
Occasionally pushing and/or pulling objects weighing 30 pounds or more
Ability to move about
Bachelor's degree in biology, chemistry or related field
Some related post-undergraduate work experience preferred
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: Commensurate with experience Employee group: Full Time Schedule: M-F, 8 hr/day Exempt Status: Non-Exempt Location: Johns Hopkins Bayview Department name: SOM DOM Pulmonary Personnel area: School of Medicine
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
The successful candidate(s) for this position will be subject to a pre-employment background check.
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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