The Dalton Laboratory is seeking a Research Technologist to conduct research into the biologic and therapeutic implications of spliceosome mutations in myeloid malignancies (acute myeloid leukemia and myelodysplastic syndromes). The laboratory uses gene editing to engineer and then study unique human cell models of cancer-associated mutations in spliceosome genes and validates findings in primary human patient samples. Biological studies include analysis of mutation-induced RNA splicing, proteome changes, and diverse cellular pathway alterations including those in energy metabolism. Therapeutic studies include targeting metabolic weaknesses, characterizing therapeutic antibody targets, and developing novel immunotherapeutic modalities against spliceosome-mutant cells. Under the general supervision of a faculty investigator or post-doctoral fellow as well as semi-independent work, the Research Technologist is responsible for laboratory research using established procedures and protocols.
Specific Duties & Responsibilities
Uses molecular biology methods (cloning, PCR assays, mutagenesis), cell culture methods (transfections, virus infections, cell line maintenance), and biochemical procedures (protein electrophoresis, western blotting, immunoprecipitation, protein purification, protein microarray, immunofluorescence).
Applies analytical skills to implement assigned projects.
Maintains accurate and current data records.
Maintains freezer stock and supply inventory.
Responsible for maintenance of lab and equipment.
Identifies and trouble-shoots methods and experimental procedures.
Special Knowledge, Skills & Abilities
DNA & RNA handling, Western blotting, PCR, cell culture.
General computer skills.
Exercises independent judgment.
Good oral and written communication skills are required.
Bachelor's Degree in Biology, Chemistry or related field.
Some related post-undergraduate work experience preferred.
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.00 - $20.40/hr (Commensurate with experience) Employee group: Full Time Schedule: M-F 8am- 4:30pm Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10002935-SOM Onc Hematologic Malignancies Personnel area: School of Medicine
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
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