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Posted: 15-Jan-23
Location: Ann Arbor, Michigan
Salary: Open
Internal Number: 229121
In addition to applying to this position through the UM Careers Website, please provide curriculum vitae, a brief summary of research accomplishments and future goals, and the names and contact information of three references to: Dr. Yanzhuang Wang, LSA Department of Molecular, Cellular and Developmental Biology, University of Michigan, 1105 North University Avenue, Ann Arbor, MI 48109-1085. Email directly to yzwang@umich.edu with subject line Research Laboratory Technician Associate
Dr. Yanzhuang Wang's laboratory is searching for a Research Laboratory Technician Associate. Research in the lab at the University of Michigan in the department of Molecular, Cellular, and Developmental Biology focuses on the biogenesis, function, and defects of the Golgi in diseases. The Golgi apparatus is a central membrane organelle for protein trafficking, glycosylation, and secretion in all eukaryotic cells. A unique feature of this organelle is a stack of flattened cisternae. Our research aims to understand how this structure is formed, why its formation is important for Golgi function under normal conditions, and how the Golgi becomes defective in its structure and function under stress or disease conditions. The work will provide a basic understanding of Golgi function in protein trafficking, glycosylation, inheritance during the cell cycle, and pathology in diseases such as Alzheimer's disease, cancer and diabetes. You will report to Dr. Yanzhuang Wang directly. You will be working full time in-person in a research lab located in Ann Arbor, Michigan. For reference, please go to our website at: https://sites.lsa.umich.edu/wang-lab/.
The Department of Molecular, Cellular, and Developmental Biology conducts basic research about the function of all branches of life bacteria, plants, and animals. The research portfolio includes more than 40 sponsored awards, amounting to more than $8 million. We are also home to the more 300 students each year who graduate with majors in molecular, cellular, and developmental biology; plant biology; biology, health and society; and microbiology.
- Conduct an independent research project on the structure and function of the Golgi apparatus
- Support lab researchers on related research. Help with some functional management and training of junior lab personnel
- Contribute to the lab workflows, including biosafety, supplies, and organization of protocols
- Manage general lab organization. Maintain accurate, organized, and clear records and protocols that can be accessed and used by the PI and research team. May help prepare and update regulatory protocols (such as animal protocols, IRB, IBC, and EHS)
- Candidates must have a high school diploma or Associate’s degree and 1 year of full-time bench lab experience in a cell biology lab.
- Basic knowledge in cell biology and biochemistry, in particular membrane biology.
- Must have experience with many basic molecular and cellular biology techniques in a research laboratory setting. Familiar with the following techniques:
*Cell culture, including transfection, sample preparation and protein concentration measurement.
*Western blotting, including casting gels, loading samples, running gels, transfer, block and incubation of membranes with antibodies, acquiring images and quantitation of results.
*Immunofluorescence microscopy, including sample preparation, imaging and quantitation. - Proficient in Microsoft Excel (EXP: can maintain complex spreadsheets of data quantification and create graphs) Photoshop, and similar software to organize and analyze experimental data.
- Transmission electron microscopy (TEM), including sample preparation of tissue culture cells and animal tissues, imaging and quantitation.
- Some prior experience working with laboratory mice or rats and animal tissue immunocytochemistry is preferred.
This position is a term-limited position for one year. There is a possibility for renewal depending on performance and availability of funding.
As one of the world’s great liberal arts colleges, LSA pushes the boundaries of what is understood about the human experience and the natural world, and we foster the next generation of rigorous and empathetic thinkers, creators, and contributors to the state of Michigan, the nation, and the world.
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