The purpose of our laboratory research in the Department of Genetic Medicine is to elucidate molecular disease mechanisms and test therapies for a group of rare diseases that disrupt epigenetics and chromatin. We are seeking a Sr. Research Specialist who will do the following. Independently work in a translational epigenetics/chromatin research lab using mouse models and human stem cell-based culture models. Identify, develop, and optimize assays and protocols. Design independently implements experimental approaches for research projects. Perform a wide range of sophisticated assays, including preparation of samples for next-generation sequencing approaches (i.e., RNA-seq, ChIP-seq, whole genome bisulfite sequencing, and/or similar methods). Requires significant and important contributions to various aspects of research efforts, including troubleshooting, independent decision-making, and training/guidance of other lab personnel including students, post-doctoral fellows, and/or staff. Contribute to writing of manuscripts and creating figures for publication and grants.
Specific Duties & Responsibilities
Scientific Responsibilities (90%)
Basic laboratory methods/techniques associated with genetics and molecular biology such as: isolation of DNA and RNA, PCR, quantitative real-time PCR, DNA electrophoresis, DNA cloning, DNA sequencing, quantitative and qualitative analyses of DNA, RNA, and protein molecules, as well as other functional and enzymatic assays.
Independently performs various higher-level assays, including Chip-qPCR and library preparation for the next-generation sequencing techniques of RNA-seq, ChIP-seq, and/or whole genome-bisulfite sequencing.
Performs basic and advanced cell culture techniques, including maintaining and differentiating induced pluripotent stem cell-derived models.
Prepares data for and performs sophisticated statistical analyses.
Uses light/fluorescence/confocal microscope and computer programs to analyze data.
Prepares buffers and solutions/reagents using knowledge of chemistry formulation.
Contributes to the maintenance of the laboratory, equipment, and supplies; conducts routine safety tests.
Maintains careful records of all samples obtained and experiments performed.
Routinely and independently assesses the quality of generated data.
Presents summaries and quality metrics of data generated at regular intervals in laboratory meetings.
Animal Model Responsibilities
Independently maintains mouse colonies.
Independently performs intra-peritoneal injections and other minor procedures on mice.
Independently performs dissection of mice for anatomical and histological studies.
Harvests and prepares tissues, particularly bone and brain, for molecular and phenotypic analyses and independently performs these analyses. Includes transcardial perfusions.
Uses scientific knowledge and lab experience to make judgments and decisions. Works independently.
Makes scientific contribution to protocol development, efficiency, and productivity.
Collaboratively and independently develops new techniques.
Applies published research methods to current experimental work by investigating published protocols and discussing them with PI.
Scientifically contributes to writing of manuscripts and grants.
Process analyzed data into figures for scientific publications and presentations.
Administrative Responsibilities (10%)
Responsible for budget management. Manages budgetary duties by ensuring charges, invoices, and orders are correctly routed to the proper grant.
Responsible for daily ordering of all laboratory consumables, reagents, supplies, and equipment; maintains financial records.
Responsible for equipment inventories and maintenance.
Ensures all laboratory protocols and biosafety approvals are up-to-date.
Responsible for overall organization, upkeep, and operational elements of laboratory, functioning as a representative for the PI.
Acts as a project manager for the lab. Coordinates with all other supporting staff units including administration and IT.
Exercises independent, managerial judgment and responsibility for decisions and results.
Helps to hire, supervise, and train incoming laboratory staff and students. Currently 1 Graduate student, 1 clinical/post-doctoral fellow (subject to change).
Advises personnel on proper laboratory techniques and use of laboratory equipment.
Advises personnel on the proper maintenance of laboratory notebooks and electronic files.
Ensures the laboratory and staff comply with annual safety inspections and safety office recommendations.
Bachelor's Degree in Biology, chemistry or related field.
Four years' experience in a comparable laboratory performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
MS, or equivalent, in Biology, Chemistry or Physics or PhD.
Experience with library preparation for next-generation sequencing, including for RNA-seq, ChIP-seq, whole genome bisulfite sequencing, or equivalent epigenetic techniques.
Experience with induced pluripotent stem cells/differentiation to neuronal lineage cells and mouse work.
Knowledge of bioinformatics.
Classified Title: Sr. Research Specialist Role/Level/Range: ACRP/04/MC Starting Salary Range: $46,560-$64,100-$81,650 HRLY (Commensurate with experience) Employee group: Full Time Schedule: M-F, 8:30 to 5pm Exempt Status: Exempt Location: School of Medicine Campus Department name: SOM Genetics Institute of Gen Medicine Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
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