The Trapecar Lab at the John Hopkins All Childrens Hospital and the Institute for Fundamental Biomedical Research in St. Petersburg, Florida, is seeking a Research Specialist II who will be responsible for independent scientific and administrative activities in our laboratory. We are looking for a new team member that is highly motivated, energetic, and independent, with interest in modeling multiorgan human physiology and application of such advanced in vitro models to gain a better understanding of the fundamental origins of immunometabolic diseases. The individual requires a good understanding of the overall objectives and interests of our research, as outlined on the lab's website - www.trapecarlab.org.
Specific Duties & Responsibilities
Design and perform experiments following established procedures, including whole blood/tissue processing, ELISA, Microscopy, DNA and RNA extractions, PCR, sequencing, biomarker, and cytokine multiplex assays.
Establish and maintain new cell cultures, including human organoids and iPS cells and make protocol modifications as needed.
Operate laboratory equipment such as flow cytometer, 3D bioprinter, bio-safety cabinet, centrifuge, incubator, ultra-low temperature freezer, LN2 freezer, multi-mode plate reader, light microscope, thermocycler, and others.
Keep detailed records of all the testing and help analyze the resulting data.
Disseminate study information directly to colleagues and collaborators.
Participate in the writing of papers and presentations for meetings/conferences.
Assist with training of new colleagues on specific lab procedures.
Use databases to maintain sample inventories and related data.
Operate lab computers for data entry and maintain accurate data records.
Assist with general lab maintenance, lab safety, manage and make purchases of basic laboratory supplies and specific project necessities and maintain necessary lab inventory.
Perform necessary administrative tasks such as but not limited to grant progress reports.
Special Knowledge, Skills, or Abilities
Excellent mammalian cell or cell line culture skills.
Conversant with various analytical methodologies including but not limited to flow cytometry, ELISA/Luminex, and microscopy. If not conversant, the expectation is for the candidate to get acquainted with the technology and become independent within six months of joining.
Knowledge of human immune cell enrichment and manipulation.
Knowledge of cell-based binding assays, surface antigen and intracellular staining methodology.
Excellent record keeping, organizational, written and verbal skills.
Familiar with routine immune functional assays for human immune cell populations.
Able to set up assays and conduct technical troubleshooting analysis.
Able to analyze data and provide a critical interpretation of results.
Ability to handle multiple priorities in a fast-paced environment.
Strong interpersonal skills for interfacing with other functional groups.
Employee may remain in a stationary position (sitting or standing) for up to 40% of the time. Employee is required to operate small laboratory tools that require manual dexterity.
Prolonged vision requirements include viewing computer screen, paper reports/documents, charts and results. Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope)
Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed. Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas. Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area. Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment. Research areas may include animal, human, human products, DNA, and radiation protocols. Use of personal protective equipment may be advised or required.
May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory). May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes). In some areas workspace is limited.
Bachelor's Degree in Biology, Chemistry or related field.
Two years laboratory experience performing relevant techniques.
Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $40,100-$55,140-$70,180 Annually (Commensurate with experience) Employee group: Full Time Schedule: 8 hours/day. Work on weekends and prolonged hours might occasionally be required. Exempt Status: Exempt Location: Florida Department name: All Childrens Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
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