Multi-disciplinary lab seeks a motivated Research Specialist II to carry out research into the pathological mechanisms of glaucoma in a team environment.
Specific Duties & Responsibilities
Confocal and multiphoton microscopy of immunohistochemical studies of explanted mouse or human ocular tissues.
Write and prepare documentation for protocols, and manuscript publication.
Complete several glaucoma model and optic nerve ophthalmic surgeries and intraocular injections in mice and rats, preparing and dosing animals in drug studies, measuring intraocular pressures via rebound tonometry, using injectable and/or gas anesthetics via commercial rodent anesthesia machine in accordance with USDA and AALAC.
Perform various biochemical techniques (western blotting, PCR), and molecular and cell biological methods (RNA and DNA isolation, sterile cell culture assays).
Histological methods performed in this position include cryopreservation and epoxy preparation (embedding and sectioning) and subsequent immunohistochemical and/or morphologic studies of animal and human donor tissues.
Image capture, reconstruction, and data analysis for morphologic study using Imaris, ImageJ, Neurolucinda, and Metamorph software.
Management of multiple mouse lines, including tailing and PCR for genotyping and maintaining database of mouse lines.
Execute proper disposal of biohazardous materials, maintain laboratory conditions and other duties as assigned by lab manager.
Prepare and carry out perfusion fixation of rats or mice, with micro-dissection of ocular and/or brain tissues.
Bachelor's Degree in biology, chemistry or related field.
Two years laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
One-to-two-year experience in a cell biology, molecular biology or neuroscience laboratory performing relevant techniques is desired.
Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $40,100-$55,140-$70,180 Annually (Commensurate with experience) Employee group: Full Time Schedule: Monday-Friday, 8:30 - 5:00 Exempt Status: Exempt Location: School of Medicine Campus Department name: SOM Oph Glaucoma Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
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