We are seeking aResearch Specialist who will employ cultured human induced pluripotent stem cells (iPSCs) from a range of neurological diseases including amyotropic lateral sclerosis and various cell types derived from these iPSCs such as cortical and spinal neurons. The Research Specialist will become proficient in the novel and modern methods of iPSC derivation and cell differentiation. We are looking for a proficient research laboratory specialist with an understanding of techniques and a solid foundation in the underlying science, gained from education and experience. The individual will work independently, manage daily research projects, be responsible for routine troubleshooting, and assist in the training and guidance of other lab personnel.
The Research Specialist will participate in planning experiments and evaluating/interpreting results utilizing detailed knowledge of research laboratory procedures and practices. The individual should be capable of independent judgment in the selection, development, performance and evaluation of research methods, and operation of appropriate equipment.
Specific Duties & Responsibilities
Follows established procedures or protocols and utilizes aseptic technique to perform laboratory experiments which include mammalian tissue culture (passaging and cryopreservation), conventional and real-time PCR, electrophoretic separations (agarose and acrylamide gels), cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.
Plans and executes a defined set of experiments following discussion with the principal investigator.
Uses various software applications, such as Excel spreadsheets, email, statistical packages, and graphics packages to assemble and/or format data and/or test reports.
Participates in the organization and operation of the laboratory.
Performs general lab maintenance duties, may need to order and manage supplies and reagents of the lab.
Maintains excellent written records and electronic databases.
Prepares solutions and reagents following standard laboratory formulas and procedures.
Trains new students and technicians
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
Completes annual university biohazard/universal precaution safety training, as appropriate.
Monitors biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.
Monitors inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.
Keeps current knowledge of vendors and suppliers of materials specific to the laboratory requirements.
Minimum Qualifications
Bachelor's Degree in biology, chemistry or related field.
One year laboratory experience performing relevant techniques.
Preferred Qualifications
Some knowledge of iPSC handling and differentiation preferred.
Some knowledge with aseptic neuronal culturing preferred.
Experience with immunohistochemistry, and basic cell and molecular biology techniques as well as reparation of standard laboratory buffers and other chemicals needed for culturing preferred.
Background in tissue culture, particularly iPSC culture preferred
Classified Title: Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: $16.61-$22.83-$29.04 HRLY (Commensurate with experience) Employee group: Full Time Schedule: Monday-Friday, 8:30am-5:00pm Exempt Status: Non-Exempt Location: School of Medicine Campus Department name: Neurodegenerative and Injury Program Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
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All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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