We are seeking a Research Specialist II, for the growing new laboratory of Prof. Greg Newby in the Department of Genetic Medicine, which is focused on the development and application of cutting-edge genome editing tools to mammalian cell and mouse models of genetic disease. The Research Specialist II will be responsible for optimizing and conducting molecular biology, cell culture, and mouse model experiments. Prior experience in genome editing methods is not required and training will be provided on the job. However, the applicant should be experienced in molecular biology and genetics research and work well with collaborations and teams. The applicant should be comfortable with identifying, developing, and optimizing molecular biology assays and working in mouse and cell culture BSL2+ facilities.
Specific Duties & Responsibilities
Scientific Responsibilities (85%)
Performs and optimizes sophisticated laboratory methods associated with genetics and molecular biology such as: mammalian iPS cell culture, isolation of DNA and RNA, digital or quantitative real-time PCR, DNA electrophoresis, DNA cloning, next-generation sequencing, and viral vector production and delivery.
Independently performs and optimizes various higher-level assays, including Chip-qPCR and library preparation for RNA-seq, ChIP-seq, and whole genome sequencing.
Maintains mouse colonies; genotyping/weighing of mice.
Mouse treatment with nanoparticle / viral vectors.
Dissection of mice for anatomical, histological, and nucleotide sequencing studies.
Uses scientific knowledge and lab experience to make judgments and decisions that may alter research objectives.
Makes scientific contribution to protocol development, efficiency, and productivity.
Collaboratively and independently develops new techniques.
Applies published research methods to current experimental work by investigating published protocols and discussing them with PI.
Administrative Responsibilities (15%)
Records research activities so that they can be clearly reproduced and interpreted by other laboratory members.
Process analyzed data into figures for scientific publications and presentations.
Communicates with collaborators to plan experiments and discuss results.
Contributes to writing peer-reviewed manuscripts and grant proposals.
Special Knowledge, Skills, and Abilities
Experience with molecular biology (including advanced) techniques, cell culture, and mouse work (as above).
Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies and laboratory experience: biology, biochemistry, chemistry, physics, microbiology and/or genetics.
Must be able to work in BSL2+ cell culture and mouse facilities.
Bachelor's degree in biology, chemistry or related field
Two years laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula
Classified Title: Research Specialist II Job Posting Title (Working Title): Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: Min $40,900 Max $71,600 ($56,250 targeted; Commensurate with experience) Employee group: Full Time Schedule: M-F/9 to 5:30 pm Exempt Status: Exempt Location: School of Medicine Department name: Genetic Medicine Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
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