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We are seeking a Research Technologist who is needed for the Coyne lab. The lab uses induced pluripotent stem cell (iPSC) derived neuron (iPSN) and postmortem human tissue models to understand the mechanistic basis and pathophysiologic consequences of impaired nuclear pore complex (NPC) and nuclear envelope surveillance and homeostasis in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).

The Research Technologist will assist with various projects in the lab and primarily be responsible for the maintenance and differentiation of iPSNs, processing of samples for western blot and qRT-PCR and carrying out western blots and qRT-PCR experiments. The technologist may also assist with immunostaining and confocal imaging as required.

The Research Technologist will participate in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. The individual should be capable of judgment in the selection, development, performance and evaluation of research methods and operation of appropriate equipment.

Specific Duties & Responsibilities 

• Follows established procedures or protocols and utilizes aseptic technique to perform laboratory experiments which include: iPSN culture (passaging and maintenance), qRT-PCR, western blotting, cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.
• Uses various software applications, such as Excel spreadsheets, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble and/or format data.
• Participates in the organization and operation of the laboratory.
• Performs general lab maintenance duties such as monitoring of inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.
• Maintains excellent written records and electronic databases.
• Prepares solutions and reagents following standard laboratory formulas and procedures.
• Plans and executes a defined set of experiments following discussion with the principal investigator.
• Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
• Completes annual university biohazard/universal precaution safety training, as appropriate.
• Monitors biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.

• Bachelor's Degree in Biology, Chemistry or related field.

• Some knowledge of or experience with aseptic mammalian cell culturing preferable but not required.
• Basic understanding of or experience with western blotting, qRT-PCR, or immunostaining preferable but not required.
• Some related post-undergraduate work experience preferred.

Classified Title: Research Technologist 
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: Min $15.50 - Max $26.00 HRLY ($36,000 targeted; Commensurate with experience) 
Employee group: Full Time 
Schedule: M-F, 8:30am-5:00pm 
Exempt Status: Non-Exempt 
Location: School of Medicine Campus 
Department name: ​​​​​​​Neurodegenerative and Injury Program  
Personnel area: School of Medicine 

Total Rewards
The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/

Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.

Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

EEO is the Law
Learn more:
https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf

Accommodation Information

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit accessibility.jhu.edu.

Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. The COVID-19 vaccine does not apply to positions located in the State of Florida. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit https://www.hopkinsmedicine.org/coronavirus/covid-19-vaccine/ and all other JHU applicants should visit https://covidinfo.jhu.edu/health-safety/covid-vaccination-information/.

The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Note: Job Postings are updated daily and remain online until filled.

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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