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Lab Technician - Dept of Molecular Biology & Genetics - College of Arts & Sciences (CAS)Technician II

The Lab Technician will assist in both the Aravin Lab, and the Fejes Toth Lab.

The Aravin Lab researches genomic parasites called transposons. If unchecked, transposons wreak havoc, jumping from place to place in the genome, causing mutations in normal genes. However, cells have a remarkable defensive system that uses non-coding RNAs as guides to identify and represses genome invaders. In animals, a distinct class of non-coding RNAs called piRNAs suppresses transposons in germline cells, ensuring fertility and the faithful transmission of genetic material through generations. The piRNA pathway works through an elegant RNA- and chromatin-based circuit and is amazingly powerful at discriminating transposons from 'good' genes and at repressing invaders in the nucleus and the cytoplasm using different mechanisms.

The Fejes Toth Lab is investigating the role of chromatin and RNA in controlling transcriptional and post-transcriptional gene regulatory processes. We are focusing on gaining mechanistic insights into how small non-coding RNAs induce changes in chromatin and transcription in animals. We are also aiming to identify the role of chromatin on post-transcriptional gene regulatory processes. We use an interdisciplinary approach combining biochemistry, genetics, cell, molecular and computational biology and functional genomics as key tools to tackle these exciting questions.

The Lab Technician provides assistance to both the Aravin Lab, and the Fejes Toth Lab. Responsibilities include but are not limited to: General lab maintenance, ordering, and organization. Maintaining fruit-fly (Drosophila) strains collection. Assisting the laboratory heads, postdocs and graduate students in conducting research experiments using techniques such as genetic crosses between defined Drosophila strains, maintaining cell cultures, basic molecular biology techniques (DNA and RNA isolation from Drosophila stocks as well as bacterial cell culture, bacterial cell transformation, plasmid DNA preparation), PCR amplification of genomic and plasmid DNA, cloning in bacterial cells (including Gibson Assembly), and light and fluorescent microscopy.

Complete training on lab safety and proper handling of chemicals as required by CALS EHS. Prepare laboratory waster for disposal per protocols. Document laboratory procedures. Assist in evaluating lab methods, procedures and techniques based on written instruction. Assist with inventory and ordering of supplies as directed.

This is a full-time (39 hours/week), benefits eligible, one-year term appointment. There is a possibility for extension based upon available work, funding, and performance.

Required Qualifications:

• Associate's degree or other formal training program of two years or equivalent; more than 1 year of education and experience.
• Must be personable, flexible, proactive, demonstrating a positive "can do" attitude, possess excellent organizational and communication skills and take pride in their work.
• Ability to develop effective working relationships with students, faculty and staff.
• Ability to maintain a comprehensive, up to date and accurate research lab notebook.
• Visual concentration, attention to detail and manual dexterity are required.
• Must be willing to learn new laboratory skills.
• Must be able to work independently after sufficient training, as well as in a team environment to accomplish required tasks.
• Must be comfortable working with Drosophila (fruit flies).
• Should be comfortable with basic computer programs such as MS Word and Excel and Google Docs.
• Familiar with basic molecular biological, cell biological, genetics and biochemical techniques.
• Able to work under general supervision and proceed alone on regular tasks.
• Experience modeling values that support inclusion, belonging, and wellbeing.

Preferred Qualifications:

• Bachelor's degree in biology, genetics, molecular biology, biochemistry, or related subjects.
• Knowledge of genetics and/or molecular biology and basic bioinformatics a plus.

Your complete application will include BOTH cover letter and resume. We have found it most efficient to save as one document prior to attaching. Review of applications will begin immediately.

No visa sponsorship or relocation assistance are provided for this position.

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